Review




Structured Review

Sangon Biotech plasmid pet30a pol β
Plasmid Pet30a Pol β, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/cas9+plasmid+puromycin+recombinant+sgrna/pmc12781883-61-0-40
Average 86 stars, based on 1 article reviews
plasmid pet30a pol β - by Bioz Stars, 2026-09
86/100 stars

Images

Related Articles

Sequencing:

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1.
Article Snippet: .. The coding sequence ofHc-SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb-SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express rHc-SPII8 or rNb-SPI-I8, induced by 1mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1
Article Snippet: .. The coding sequence of Hc -SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb -SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express r Hc -SPI-I8 or r Nb -SPI-I8, induced by 1 mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Polymerase Chain Reaction:

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1.
Article Snippet: .. The coding sequence ofHc-SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb-SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express rHc-SPII8 or rNb-SPI-I8, induced by 1mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1
Article Snippet: .. The coding sequence of Hc -SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb -SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express r Hc -SPI-I8 or r Nb -SPI-I8, induced by 1 mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Amplification:

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1.
Article Snippet: .. The coding sequence ofHc-SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb-SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express rHc-SPII8 or rNb-SPI-I8, induced by 1mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1
Article Snippet: .. The coding sequence of Hc -SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb -SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express r Hc -SPI-I8 or r Nb -SPI-I8, induced by 1 mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Clone Assay:

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1.
Article Snippet: .. The coding sequence ofHc-SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb-SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express rHc-SPII8 or rNb-SPI-I8, induced by 1mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.

Article Title: Nematode serine protease inhibitor SPI-I8 negatively regulates host NF-κB signalling by hijacking MKRN1-mediated polyubiquitination of RACK1
Article Snippet: .. The coding sequence of Hc -SPI-I8B (without signal peptide and TIL domain) was PCR amplified from H. contortus cDNA and inserted into pET32a plasmids (cat no. A339132, Sangon Biotech), whereas the coding sequence of Nb -SPI-I8 was cloned into pET30a plasmids (cat no. B540185, Sangon Biotech). .. Recombinant plasmids were transformed into competent cells to express r Hc -SPI-I8 or r Nb -SPI-I8, induced by 1 mM isopropylthio-β-galactoside (IPTG) at 16 °C for 24 h. Recombinant protein was isolated from bacteria using a sonication method, then purified using Ni-NTA agarose column (cat no. 30250, Qiagen, Shanghai, China) according to the manufacturer’s instructions.



Similar Products

86
Sangon Biotech plasmid pet30a pol β
Plasmid Pet30a Pol β, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/cas9+plasmid+puromycin+recombinant+sgrna/pmc12781883-61-0-40
Average 86 stars, based on 1 article reviews
plasmid pet30a pol β - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

86
Sangon Biotech m2e linker ste linker pet30a plasmids
M2e Linker Ste Linker Pet30a Plasmids, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/cas9+plasmid+puromycin+recombinant+sgrna/pm41443448-47-3-8
Average 86 stars, based on 1 article reviews
m2e linker ste linker pet30a plasmids - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

93
Addgene inc hygro p2a mab
Hygro P2a Mab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB-D135S+(Plasmid+%2379051)/bio_rxiv__2025__11__05__686800-164-1-3
Average 93 stars, based on 1 article reviews
hygro p2a mab - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc pet30a alkb d135s
Pet30a Alkb D135s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB-D135S+(Plasmid+%2379051)/bio_rxiv__2025__11__05__686800-163-5-6
Average 93 stars, based on 1 article reviews
pet30a alkb d135s - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc pet30a alkb
Pet30a Alkb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB+(Plasmid+%2379050)/bio_rxiv__2025__11__05__686800-163-1-2
Average 93 stars, based on 1 article reviews
pet30a alkb - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc pet30a alkb δn11
Pet30a Alkb δn11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB+(Plasmid+%2379050)/pmc12632813-39-7-5
Average 93 stars, based on 1 article reviews
pet30a alkb δn11 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc plasmid alkb wild type protein
Plasmid Alkb Wild Type Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB+(Plasmid+%2379050)/pmc12632813-39-0-5
Average 93 stars, based on 1 article reviews
plasmid alkb wild type protein - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc plasmid d135s mutant protein
Plasmid D135s Mutant Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB-D135S+(Plasmid+%2379051)/pmc12632813-40-0-5
Average 93 stars, based on 1 article reviews
plasmid d135s mutant protein - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

93
Addgene inc alkb wild type protein
Alkb Wild Type Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet30a+plasmids/pET30a-AlkB+(Plasmid+%2379050)/pmc12632813-366-9-14
Average 93 stars, based on 1 article reviews
alkb wild type protein - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results